Live cell X-ray imaging of autophagic vacuoles formation and chromatin dynamics in fission yeast
نویسندگان
چکیده
منابع مشابه
Live Cell Imaging in Fission Yeast.
Live cell imaging complements the array of biochemical and molecular genetic approaches to provide a comprehensive insight into functional dependencies and molecular interactions in fission yeast. Fluorescent proteins and vital dyes reveal dynamic changes in the spatial distribution of organelles and the proteome and how each alters in response to changes in environmental and genetic compositio...
متن کاملCentromeric chromatin in fission yeast.
A fundamental requirement for life is the ability of cells to divide properly and to pass on to their daughters a full complement of genetic material. The centromere of the chromosome is essential for this process, as it provides the DNA sequences on which the kinetochore (the proteinaceous structure that links centromeric DNA to the spindle microtubules) assembles to allow segregation of the c...
متن کاملQuantitative Live Cell Fluorescence-microscopy Analysis of Fission Yeast
Several microscopy techniques are available today that can detect a specific protein within the cell. During the last decade live cell imaging using fluorochromes like Green Fluorescent Protein (GFP) directly attached to the protein of interest has become increasingly popular. Using GFP and similar fluorochromes the subcellular localisations and movements of proteins can be detected in a fluore...
متن کاملTopic Introduction Live Cell Imaging of Yeast
The development of cloning vectors for green fluorescent protein (GFP) and the simplicity of yeast reverse genetics allow straightforward labeling of yeast proteins in living cells. Budding and fission yeast are therefore attractive organisms in which to study dynamic cellular processes such as growth, cell division, and morphogenesis using live cell fluorescence microscopy. This article focuse...
متن کاملEvaluation of multifunctional targeted gold nanoparticles on X-ray attenuation in nasopharyngeal cancer cells by X- ray imaging
Introduction: Head-and-neck cancer is the sixth most common cancer worldwide with the number of cases consistently increasing in developing countries. Successful development of effective, safe and cost effective nanoprobes for head-and-neck cancer targeting imaging is a big challenge. This study is aimed to develop cysteamine-folate conjugated gold nanoparticles (F-Cys-AuNPs) a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Scientific Reports
سال: 2017
ISSN: 2045-2322
DOI: 10.1038/s41598-017-13175-9